2D cell culture has been the gold standard for 100 years. In the last 30 years, there has been a movement towards 3D cell culture. Growing cells in 3D allows for the study of migration, differentiation, proliferation, and morphology of cells in an environment similar to what is found in vivo.
Studying cells in an environment that more closely resembles the human body (3D), allows for more accurate and predictive results for future in vivo tests.
Collagen is the most abundant protein found in the human body. It is found in nearly every tissue and organ. Thus, it makes sense to grow a majority of cell types in a 3D collagen environment.
PureCol® (3 mg/ml), Nutragen® (6 mg/ml) and FibriCol® (10 mg/ml), are type I bovine collagen solutions, that can easily form 3D hydrogels for cell culture. Combined, they have been referenced in over 3000 publications. These products are recognized worldwide for consistency, reliability, and reproducibility.
The different concentrations of the above products (3-10 mg/ml) create hydrogels of different rigidities, allowing for the customization of experiments.