Lifeink® 500 Kit

Calcium Phosphate Bioink

Catalog Number: #5231-1KIT

Lifeink® 500 contains 5 mL of calcium phosphate cement. The paste, after printing, will harden and become a calcium deficient, porous, hydroxyapatite material to serve as a bone or hard tissue substitution scaffold.

This product is now exclusively available through Innotere Biomaterial. Click the Order Now button to be redirected to their website.

Print complex geometries and scaffolds for advanced cell culture or animal models.

Special thanks to INNOTERE biomaterial for their collaboration, data, and images to help bring Lifeink® 500 to researchers worldwide.

Properties of Lifeink® 500

  • Self-Setting to form final solid material

  • Extruded filament fidelity and continuity

  • Biocompatible and bioresorbable bone or hard tissue substitution material

  • Chemically stable in neutral or alkaline medium

  • High compressive strength up to 30 MPa allows for long term experiments

  • Infill printed scaffolds with collagen hydrogels for enhanced cell seeding

  • Long processing time

Lifeink® 500 Scaffold Wettability and Cell Encapsulation

Lifeink® 500 serves as a great bioink for 3D printing cell culture scaffolds.

  • 3D print scaffold

  • Mix your cells with neutralized Type I collagen

  • Dispense the collagen/cell suspension over the printed scaffold

  • Incubate at 37°C to polymerize your collagen scaffold, and encapsulate your cells within the hydroxyapatite/collagen matrix.

Lifeink® 500 Mechanical Loading

3D bioprinted calcium phosphate scaffolds were implanted in sheep to study how these scaffolds augment bone formation. Image to the right (top) are 6-week x-rays showing in-tact scaffolds.

 

 

 

 

 

 

 

After 6 weeks, new bone covered nearly 60% of the porous walls of the scaffolds, as seen to the right (bottom).

Reitmaier et al.: Strontium(II) and Mechanical Loading Additively Augment Bone Formation in Calcium Phosphate Scaffolds; Journal of Orthopaedic Research 2017

Product Specifications

The final ​material consists of synthetic calcium phosphates, mainly alpha-tricalcium phosphate and microcrystalline (calcium deficient) hydroyapatite.

  • Appearance                              Off-white, opaque paste

  • Package Size                            5 mL

  • Gel Consistency                        No apparent bubbles

  • Extrudability                              Extrudes through 22 gauge                                                       needle.

  • Crosslinking                              Self-setting process

  • Storage                                      Dry at room temperature

Print Recommendations

  • Dispensing tip diameter: 0.3-0.8 mm, tapered or cylindrical

  • Needle length: Shorter the better (<20 mm)

  • Print head speed: 1-10 mm/s

  • Pressure: 30-80 PSI (depending on print speed)

  • Print on glass

  • Induce cement setting by incubating scaffolds in water-saturated atmosphere, or immerse in aqueous liquid for 3 days.

  • Wash and dry scaffolds in acetone

  • Dry scaffolds can be gamma-sterilized or autoclaved

Published References

  • Klein, A. et al. Effect of bone sialoprotein coated three-dimensional printed calcium phosphate scaffolds on primary human osteoblasts. Journal of Biomedical Materials Research Part B: Applied Biomaterials (2018). doi:10.1002/jbm.b.34073

  • Lode, A. et al. Strontium-modified premixed calcium phosphate cements for the therapy of osteoporotic bone defects. Acta Biomaterialia 65, 475–485 (2018).

  • Reitmaier, S. et al. Strontium(II) and mechanical loading additively augment bone formation in calcium phosphate scaffolds. Journal of Orthopaedic Research (2017). doi:10.1002/jor.23623

  • Akkineni, A. R. et al. 3D plotting of growth factor loaded calcium phosphate cement scaffolds. Acta Biomaterialia 27, 264–274 (2015).

  • Moussa, M. et al. Medium-Term Function of a 3D Printed TCP/HA Structure as a New Osteoconductive Scaffold for Vertical Bone Augmentation: A Simulation by BMP-2 Activation. Materials 8, 2174–2190 (2015).

  • Durual, S. et al. A 3D-printed TCP/HA osteoconductive scaffold for vertical bone augmentation. Dental Materials 31, (2015).

  • Lode, A. et al. Fabrication of porous scaffolds by three-dimensional plotting of a pasty calcium phosphate bone cement under mild conditions. Journal of Tissue Engineering and Regenerative Medicine 8, 682–693 (2012).

  • Luo, Y., Lode, A., Sonntag, F., Nies, B. & Gelinsky, M. Well-ordered biphasic calcium phosphate–alginate scaffolds fabricated by multi-channel 3D plotting under mild conditions. Journal of Materials Chemistry B 1, 4088 (2013).

For additional information such as directions for use, SDS, material origin, or certificate of analysis, click here.

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