Bioprinting Bioglass Initial Study and Results

The purpose of this study was to evaluate the printability of Bioglass mixed with Lifeink® 200 collagen bioink. 

 

The bioglass creates a bioactive environment to promote hydroxyapatite formation and osteoblast bone secretion.

60% bioglass/40% collagen is the desired concentration, in order to best match the physiological ratios of inorganic/organic materials found in vivo.

This work was done in collaboration with Dr. Vipuil Kishore and his graduate student Mr. Nilabh Kajave from the Functional Biomaterials and Tissue Engineering Laboratory at Florida Institute of Technology.

Alizarin Red S Staining

Alizarin Red S staining was performed on Lifeink® 200 collagen filaments that had been extruded through a 30 gauge needle into 1X PBS. The filaments had 0, 10, or 60% wt/wt bioglass. Red stain confirms bioglass incorporation in the filaments.

Scale bar indicates 200 um.
 

0% Bioglass

10% Bioglass

60% Bioglass

Alignment of Bioprinted Collagen

Polarized microscopy was used to determine the amount of alignment evident in Lifeink® 200 extruded filaments. Lifeink® 200 was mixed with bioglass in different amounts (0, 10, or 60% wt/wt bioglass) and extruded through a 30 gauge needle. The light blue color indicated aligned collagen fibers. Results indicate some partial collagen fiber alignment in the collagen/bioglass filaments.

 

Fully aligned collagen control

10% Bioglass

60% Bioglass

SEM Imaging for Bioglass Incorporation

SEM imaging was performed on Lifeink® 200 extruded filaments to confirm the presence of bioglass post-printing in the 60% wt/wt bioglass ink. Results show bioglass incorporated into the printed collagen filaments. 
 

0% Bioglass 1000X

60% Bioglass 1000X

0% Bioglass 3000X

60% Bioglass 3000X

SEM Imaging - Hydroxyapatite Formation

SEM imaging was performed on Lifeink® 200 extruded filaments to confirm the formation of hydroxyapatite after 7 days incubation in simulated body fluids (SBF). Results show an evident layer of hydroxyapatite on the 60% wt/wt bioglass/collagen bioink extruded filaments.
 

0% Bioglass 3000X

10% Bioglass 3000X

60% Bioglass 3000X

3D Bioprinting Protocol

For the protocol to mix bioglass with Lifeink® 200 for 3D bioprinting, please contact support@advancedbiomatrix.com. 

 

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