Lifeink® 200 Kit

Catalog Number: #5202-1KIT

Lifeink® 200 contains 5 mL of highly purified, pH neutral collagen formulated in physiological salts. Cells can be directly added and uniformly mixed in the bioink and then extruded via a 30-gauge needle or larger.

Lifeink® 200 allows for high resolution printing and stable long-term structures when printing into the FRESH support slurry.

Price: $230

Highly Concentrated Type I Collagen

Kit Contents:

  1. 5 mL concentrated type I collagen bioink (1/kit)

  2. Syringe couplers (2/kit)

  3. Syringe caps (2/kit)

  4. Sterile 10 mL syringe (1/kit)

Print complex living tissues or simple constructs with

Lifeink® 200, the only pure type I collagen bioink in the world.

Meniscus

Meniscus

Bioprinted with Lifeink 200 in FRESH.

Scaffold

Scaffold

Bioprinted with Lifeink 200 in FRESH.

Nose

Nose

Bioprinted with Lifeink 200 in FRESH.

Ear

Ear

Bioprinted with Lifeink 200 in FRESH.

Tubes

Tubes

Bioprinted with Lifeink 200 in FRESH.

Print Properties of Lifeink® 200

  • Native extracellular matrix proteins

  • High print resolution

  • Extruded filament fidelity and continuity

  • Shear thinning and recovery (thixotropic) 

  • Thermal crosslinking at 37°C

  • Printable between 4-25°C

  • Mechanical construct strength

  • Physiological pH and salt concentration

  • Cell friendly and biocompatible

  • Sterility and endotoxin tested

Dermal fibroblasts in Lifeink® 200

Lifeink® 200 Cell Culture

Fibroblasts, day 1

10M Cells/mL, 10X magnification

Adipose Derived Stem Cells, day 1

10M Cells/mL, 10X magnification

Fibroblasts, day 6

10M Cells/mL, 10X magnification

Adipose Derived Stem Cells, day 6

10M Cells/mL, 10X magnification

3D Printed Lattice

eGFP expressing chondrocytes were encapsulated within Lifeink® 200 and 3D bioprinted. 

Video demonstrates high cell viability after 2 days of cell culture. Images were taken at 10X through approximately 350 um. 

Special thanks to Thomas Kean for the bioprinting work, Danielle MacKay for imaging. We gratefully acknowledge the Integrated Microscopy Core at Baylor College of Medicine for equipment use and the Rolanette and Berdon Lawrence Bone Disease Program for funding.

Lifeink® 200 Shear Thinning and Recovery

A sample of Lifeink® 200 was tested on a Bohlin rheometer with gradually increasing strain for 5 minutes. The sample was then immediately rested, a total of 4 times. Results indicate that Lifeink® 200 demonstrates the unique properties of both shear thinning, and recovering.

Lifeink® 200 Kinetic Gel Stiffness

Lifeink® 200 undergoes an increase in gel stiffness upon incubation at 37°C for >10 minutes. This helps further crosslink and solidify printed constructs.

Lifeink® 200 Flex Test

Constructs printed with Lifeink® 200 act like real tissues. Watch how this printed net will flex like a tissue, and then recover to its original shape once the stimuli is removed.

Want to modify Lifeink® 200?

Here are some helpful application notes:

Mixing in additional ECM's to Lifeink® 200

This protocol explores how to customize Lifeink® 200 with extra purified ECM's to best replicate the tissue that you want to print.

Mixing Lifeink® 100 and Lifeink® 200

This protocol shares how to mix the methacrylated collagen with the high concentration collagen, allowing for high resolution prints in FRESH that can be UV crosslinked. 

Using Riboflavin to UV-Crosslink Lifeink® 200 Scaffolds

This protocol teaches how to use riboflavin and UV crosslinking to provide additional strength to bioprinted Lifeink® 200 collagen scaffolds.

Mixing Lifeink® 200 with Lifeink® 400

This protocol teaches how to mix our type I collagen with methacrylated hyaluronic acid in order to create tissues that more closely resemble the human body, and can be UV/Visible light crosslinked for additional strength.

Product Specifications

Our extensive list of product specifications for Lifeink® 200 ensure lot-to-lot consistency, and reliable and reproducible results. 

  • Appearance                              Off-White, Opaque Gel

  • Package Size                            5 mL

  • Syringe                                      BD 10 mL

  • Gel Consistency                        No apparent bubbles

  • Extrudability                              Extrudes via 30 gauge needle                                                             with pressures < 35 PSI

  • pH                                               6.8 - 7.4

  • Stress Response                      Shear-Thins and Recovers

  • Purity                                         > 98% collagen

  • Bioprintability                           160 um resolution in FRESH

  • Collagen Content                       High concentration

  • Biocompatibility                        Supports cell growth

  • Sterility                                      No growth

  • Endotoxin                                  < 10 EU/mL

  • Crosslinking                             Thermal

For additional information such as directions for use, SDS, material origin, or certificate of analysis, click here.

Lifeink® 200 Frequently Asked Questions

Can I print Lifeink® 200 with the FRESH method?


We highly recommend using the FRESH method to print Lifeink® 200. It gives you the ability to print high resolution, complex structures or tissues, without having to fill your ink with non-native materials (such as alginate or cellulose).




Is Lifeink® 200 really only collagen?


Yes! Lifeink® 200 is a 35 mg/ml concentration collagen bioink, comprised of ~97% type I and ~3% type III collagen.




Can I mix or add other materials with Lifeink® 200?


Yes! The Directions for Use document provides videos showing how to mix in media, proteins, or cells with Lifeink® 200.




Is Lifeink® 200 photocrosslinkable?


Contact us at Support@advancedbiomatrix.com to discuss possible options.




After printing in FRESH, how do I culture my collagen tissue?


After the print is complete, simply incubate your dish containing the FRESH slurry/encapsulated printed construct at 37°C. The FRESH gelatin microparticles will melt, and your collagen will polymerize. Once the collagen structure is released and floating in the melted gelatin, simply replace the gelatin with warm cell culture media. For example, pipette out 2 mL of melted slurry and replace with 2 mL of media. Repeat until the gelatin is fully washed out.





Need Basic Printing Parameters to Get Started?

Use these parameters as a starting point. Individual printers and bioink formulations (ie. protein concentration, cell density/volume, etc..) will require modified parameters. Hover over a printer to see our recommended print settings. 

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