Lifeink® 100 Kit

Methacrylated Type I Collagen for UV-Crosslinkable Prints

Catalog Number: #5204-1KIT

Lifeink® 100 contains 100 mg of purified methacrylated Type I bovine collagen in a kit, which provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness. Lifeink® 100 works best when printed into the FRESH support slurry.

Price: $300

Kit Contents:

  1. 100 mg lyophilized methacrylated type I collagen (1/kit)

  2. 20 mM acetic acid solution (50 mL)

  3. Neutralization Solution (10 mL)

  4. Photoinitiator - Irgacure 2959 (100 mg)

  5. Syringe couplers (2/kit)

  6. Syringe caps (2/kit)

  7. Sterile 10 mL syringe (2/kit)

Not bioprinting? Lifeink® 100 is also available as a reagent (PhotoCol®) for 3D hydrogels and cell culture. Click here to visit the PhotoCol® page.

Print type I collagen constructs that can be crosslinked with UV or visible light for increased structural strength with Lifeink® 100.

Print Properties of Lifeink® 100

  • Native extracellular matrix proteins

  • Strong thermal crosslinking at 37°C

  • Post-print UV-crosslinking

  • Printable neutralized at 4°C

  • Printable acidified at 22°C

  • Mechanical construct strength

  • Physiological pH and salt concentration

  • Cell friendly and biocompatible

  • Sterility and endotoxin tested

Lifeink® 100 Cell Culture

Adipose Derived Stem Cells, day 1

10M Cells/mL, 10X magnification

Adipose Derived Stem Cells, day 1

10M Cells/mL, 10X magnification, UV Crosslinked

Want to modify Lifeink® 100?

Here are some helpful application notes:

Mixing Lifeink® 100 and Lifeink® 200

This protocol shares how to mix the methacrylated collagen with the high concentration collagen, allowing for high resolution prints in FRESH that can be UV crosslinked. 

Product Specifications

Our extensive list of product specifications for Lifeink® 100 ensure lot-to-lot consistency, and reliable and reproducible results. 

  • Appearance                            Lyophilized Powder

  • Package Size                          100 mg

  • Extrudability                            Extrudes via 30 gauge needle                                                          with pressures < 35 PSI

  • pH                                             6.8 - 7.4

  • UV Crosslinking                      Provides ~2-3X increased                                                                stiffness

  • Purity                                       > 98% collagen

  • Collagen Content                    Methacrylated Type I Collagen

  • Biocompatibility                      Supports cell growth

  • Sterility                                    No growth

  • Endotoxin                                < 10 EU/mL

For additional information such as directions for use, SDS, material origin, or certificate of analysis, click here.

As the most abundant protein in the body, collagen is a key component for cell culture. Collagen methacrylate can be used as a rapidly self-assembling type I collagen to form cross-linked hydrogels for tissue engineering[1]. It has been used with mesenchymal stem cells[2], fibroblasts, adipose derived stem cells, epithelial cells, and many more. Collagen methacrylate is useful for forming scaffolds with varying degree of stiffness, by altering collagen concentration or the dose of UV-light exposure[2]. It is also being extensively characterized for its usefulness in 3D bioprinting (extrusion, inkjet, and photolithographic[3]).

 

1. Isaacson, A., Swioklo, S. & Connon, C. J. 3D bioprinting of a corneal stroma equivalent. Experimental Eye Research 173, 188–193 (2018).

2. Kathryn E. Drzewiecki et al, A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine, TECHNOLOGY (2017). 

3. Drzewiecki, K. E. et al. Methacrylation Induces Rapid, Temperature-Dependent, Reversible Self-Assembly of Type-I Collagen. Langmuir 30, 11204–11211 (2014).

4. Gaudet, I. D. & Shreiber, D. I. Characterization of Methacrylated Type-I Collagen as a Dynamic, Photoactive Hydrogel. Biointerphases 7, 25 (2012).

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