Adding in additional native proteins to Lifeink® 200

A bioink needs to replicate the ideal cellular environment. There is no "one size fits all" bioink.

All of our Lifeink® bioinks are easily customized by mixing in additional native, highly purified proteins that help recreate the ideal cellular environment for YOUR cells.

Click on the desired protein below.

Native Proteins to add to Lifeink®

One of a family of proteins found particularly in the flesh and connective tissues. Type II is found predominantly in hyaline cartilage and the vitreous humor of the eye.
Provides structure and strength to connective tissue. Is found many places in the body, especially skin, lung, intestinal walls and the walls of blood vessels. 
The primary collagen found in the extracellular basement membranes separating a variety of epithelial and endothelial cells. A major component of the dermal-epidermal junction where it is mostly found in the lamina densa.
Provides structure and strength to connective tissue. Is found in many places in the body, especially skin, lung, intestinal walls and walls of blood vessels.
Primary function is related to cell adhesion and attachment to extracellular matrix. Certain domains of Fibronectin have been found to play important roles that involve interactions with collagen, heparin and other cell surface glycosaminoglycans (GAGs). 
Monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types.
Elastin is found in many tissues of the body including the skin, arterial walls and ligaments.  Elastin confers strength and elasticity to tissues and organs.

Instructions for mixing native proteins with Lifeink®

1. Add the concentrated cell suspension in cell culture media to a sterile syringe.

Video for step 1

2. Add desired neutralized protein (ie. Collagen type III) to cell suspension/cell culture media from step 1.
Note: We recommend a final bioink cell concentration of 5X10^6/mL or greater.


Note: For best results, add 2 mL of cell suspension/additional protein per 5 mL of collagen bioink. Use a similar ratio for smaller volumes.


3. Place sterile coupler on the end of the syringe with the cell suspension.


4. Slowly push plunger in until media forms a slight external meniscus above the end of the coupler on the syringe.


5. Remove cap from the syringe with collagen and slowly push plunger in until collagen forms a slight external meniscus above the end of the syringe.


6. Couple the syringe with cells to the syringe with collagen. (Ensure that there are no air bubbles in the system. The “external meniscus” on both syringes helps ensure that there are no air bubbles introduced).


7. Slowly push plungers back and forth ~40 times to ensure thorough mixing. End with all of the material in the syringe to be used for printing.


Video for steps 2-6


8. The cell-laden bioink is now ready for positive displacement printers.


Note: For pneumatic printers, transfer the collagen into an appropriate syringe using the coupler. The new syringe should have the seal inserted, but the plunger removed. Centrifuge the syringe at 2000 RPM for 1 minute after transferring the collagen to remove any air bubbles.


Watch this video for help​

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